Molecular Themes in DNA ReplicationLynne S Cox Royal Society of Chemistry, 31.10.2009 - 315 sivua DNA replication, the process of copying one double stranded DNA molecule to form two identical copies, is highly conserved at the mechanistic level across evolution. Interesting in its own right as a fascinating feat of biochemical regulation and coordination, DNA replication is at the heart of modern advances in molecular biology. An understanding of the process at both the biological and chemical level is essential to developing new techniques in molecular biology. Insights into the process at the molecular level provide opportunities to modulate and intervene in replication. Rapidly dividing cells need to replicate their DNA prior to division, and targeting components of the replication process is a potentially powerful strategy in cancer treatment. Conversely, ageing may be associated with loss of replication activity and restoring it to cells may moderate some of the diseases associated with old age. Replication is, therefore, fundamental to a huge range of molecular biological and biochemical applications, and provides many potential targets for drug design. The fast pace of replication research, particularly in providing new structural insights, has outdated the majority of available texts. This learned, yet accessible, book contains the latest research written by those conducting it. It examines conserved themes providing a biological background for biochemical, chemical and pharmaceutical studies of this huge and exciting field. Rather than simply "itemising" the replication steps and the proteins involved, replication is tackled from a novel perspective. The book provides logical groupings of processes based upon biochemical similarities. The emphasis on mechanisms and the relationship between structure and function targets the chapters towards biochemists and biological chemists as well as molecular and cell biologists. The book highlights new insights into the replication process, from the assembly of pre-replication complexes, through polymerisation mechanisms, to considering replication in the context of chromatin and chromosomes. It also covers mitochondrial DNA replication, and includes archaeal paradigms, which are proving increasingly relevant to the study of replication in higher eukaryotes. Exciting potential drug targets in DNA replication are discussed, particularly in the context of treating malaria and cancer. |
Sisältö
CHAPTER 1 Conserved Steps in Eukaryotic DNA Replication | 1 |
CHAPTER 2 The Action of AAA+ ATPases in Loading Replication Factors | 22 |
CHAPTER 3 Ring Structures and Sixfold Symmetry in DNA Replication | 47 |
CHAPTER 4 Mechanisms for High Fidelity DNA Replication | 86 |
CHAPTER 5 Coordination of Nucleases and Helicases during DNA Replication and Doublestrand Break Repair | 112 |
CHAPTER 6 Molecular Handoff Mechanisms in DNA Replication | 156 |
CHAPTER 7 Coping with DNA Damage and Replication Stress | 178 |
CHAPTER 8 Telomeres and the End Replication Problem | 217 |
CHAPTER 9 Keeping Replicated Chromatids Together Until Mitosis | 269 |
CHAPTER 10 Replication of Chromatin | 297 |
CHAPTER 11 Mitochondrial DNA Replication | 316 |
CHAPTER 12 DNA Replication in the Archaea a Paradigm for Eukaryotic Replication | 346 |
CHAPTER 13 DNA Replication in the Human Malaria Parasite and Potential for Novel Drug Development | 363 |
CHAPTER 14 Drug Targets in DNA Replication | 393 |
414 | |
Muita painoksia - Näytä kaikki
Yleiset termit ja lausekkeet
AAA+ Acad archaeal assembly ATP hydrolysis ATPase Biochem C-terminal cancer catalytic Cdc6 Cdt1 Cell Biol cell cycle cell nuclear antigen Chapter checkpoint Chem chromatin chromosome clamp loader cohesin condensin conserved Curr deletion dimer DNA binding DNA damage DNA polymerase DNA repair DNA replication DNA synthesis Dna2 domain double-strand EMBO enzyme eukaryotic exonuclease factor falciparum FEN1 Figure function G-quadruplex geminin Genes Dev Genet genome helicase activity histone homologous human telomerase inhibition inhibitor initiation interaction kinase lagging strand lesions ligase loading mammalian MCM2-7 complex mechanism mitochondrial DNA molecular molecules motif mtDNA mutations Natl nuclease Nucleic Acids Res nucleosome nucleotide Okazaki fragment pathway PCNA phosphorylation primase primer Proc processing recombination region regulation replication fork replication origins replication proteins replisome role Saccharomyces cerevisiae sequence Sgs1 single-stranded sister chromatid ssDNA subunits target telomerase RNA telomere template transcription vitro vivo yeast